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I have had my ES330 since November 2003. Within 1 month, I heard a harmonic whine when driving between 35 MPH and 60 MPH, whether I am cruising, accelerating, or foot off the gas. It appears to be getting louder since then. I cannot identify the location, but it appears to be at the rear of the car.

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It could be the tires causing this noise. Check the tire inflation and make sure they're in specification. I run my inflation at about 30psi on an '03 ES. What brand tires do you have? My car was equipped with Bridgestone tires and they're very quiet and smooth.

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